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The effect of heat shock on acute hypertrophic signaling following skeletal muscle damage
Author(s) -
Touchberry Chad D.,
Bomhoff Greg L,
Gupte Anisha,
Geiger Paige,
Gallagher Phil
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1165.6
Subject(s) - protein kinase b , heat shock protein , skeletal muscle , hsp70 , hsp27 , medicine , endocrinology , muscle hypertrophy , heat shock , soleus muscle , chemistry , phosphorylation , creatine kinase , biochemistry , gene
Heat shock proteins (Hsp's) are molecular chaperones that are critical for the maintenance of cellular homeostasis and have been shown to protect cells and tissues from a large variety of protein damaging insults. While Hsp's have been implicated in reducing damage and preventing apoptosis, it has recently been suggested that heat induction of Hsp's may inhibit hypertrophy and the rate of muscle regeneration. The purpose of this study was to determine if a single heat shock (HS) treatment influences Akt & p70s6k, potent regulators of cell growth. Wistar rats were randomized into two groups: control (core temp: 35.4°C) or heat stressed (core temp: 41°C, 20 min) 48 hours prior to a bout of downhill treadmill running (18 m/min, −16% grade). Soleus (Sol), red vastus lateralis (VLR) and white vastus lateralis (VLW) were assessed for Hsp70, Hsp27, Akt, and p70s6k 150 min following exercise. Heat shock significantly (p ≥ 0.05) increased core temperature and Hsp content in skeletal muscle, as well as significantly (p ≥ 0.05) reduced plasma creatine kinase levels. Heat shock resulted in non‐significant changes (p ≤ 0.05) in phosphorylation of the hypertrophic proteins Akt (Sol: −1.5% VLR: 61.1%, VLW: 8.1% ) & p70s6k (Sol: 6.5%,VLR: 52.7% , VLW: 35.42% ). Contrary to previous literature, our data suggests that the induction of Hsp's does not impair the acute hypertrophic response in skeletal muscle as measured by Akt & p70s6k.