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Proteomic identification of peculiar targets regulated by upstream kinases of Snf1/AMP‐activated protein kinase in yeast Saccharomyces cerevisiae
Author(s) -
Kim MyoungDong,
Cho JeaWon,
Park EunHee,
Chung ByungHee,
Kim JongDai,
Lee HyeonJu,
Kim EunAe,
Choe Myoen
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1162.8
Subject(s) - saccharomyces cerevisiae , kinase , biochemistry , ask1 , c raf , map2k7 , protein kinase a , yeast , cyclin dependent kinase 2 , glycerol kinase , biology , cyclin dependent kinase 1 , chemistry , microbiology and biotechnology , glycerol , gene , cell cycle
In budding yeast Saccharomyces cerevisiae, Snf1 (sucrose non‐fermenting) protein kinase of the Snf1/AMP‐activated protein kinase family is required for growth on non‐fermentable carbon sources and non‐preferred sugars. Three kinases, Elm1, Sak1 and Tos3, activate Snf1 by phosphorylation of its activation‐loop threonine in stress condition such as low glucose level. The absence of all three upstream kinases causes the Snf‐ phenotype. In this study, substantial role of each upstream kinase of Snf1 was explored by proteomic analysis of S. cerevisiae strains grown in glucose and glycerol. MALDI‐TOF analysis indicated that each protein kinase regulates expressions of peculiar targets even in non‐stress condition.