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MicroRNA expression profiling in placenta from a mouse model of preeclampsia
Author(s) -
Zhou Yi,
Guruju Mallikarjuna R,
Woods Ashley K,
Sharma Ram V,
Davisson Robin L
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1162.4
Subject(s) - microrna , mirbase , preeclampsia , biology , microarray , placenta , gene expression profiling , fold change , in silico , microarray analysis techniques , gene , gene expression , andrology , bioinformatics , genetics , fetus , medicine , pregnancy
Our group discovered a genetic mouse model (BPH/5) that spontaneously develops hallmarks of preeclampsia, which can be used to investigate disease mechanisms. Emerging evidence suggests that microRNAs play an important role in embryonic development. Here we tested the hypothesis that the pathogenesis of preeclampsia is associated with changes in expression of specific miRNAs in developing placenta. Microarray technology was employed for the miRNA expression profiling in BPH/5 and C57Bl/6 fetoplacental units (day 10.5). We observed differential expression of 52 miRNAs in BPH/5 fetoplacental unit vs C57B1/6 controls of which 14 were up‐regulated, and 38 were down‐regulated (fold change >2, p<0.05, n=3). Two of the differentially expressed miRNAs, mmu‐mir‐15b, mmu‐mir‐29c, were further validated by real time stem‐loop RT‐PCR (>3 fold increase in BPH/5 vs. C57, P<0.05; n=3). To understand the contributions of these up‐ or down‐regulated miRNAs to the changes in fetoplacental development in preeclampsia, the potential target genes of miRNAs were identified using three algorithms: PicTar, TargetScan and miRBase. Through computational analysis, these two miRNAs were found to be putative regulators of VEGF, PAPPA, MMP, IGF1 and CCND2, genes that are involved in feto‐placental development. Further validation of miRNA target genes with luciferase reporter assay and miRNA function analysis are in progress.