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Gene expression analysis of circulating leukocytes of rat strains exhibiting long and short survival times following severe hemorrhage
Author(s) -
Bowman Phillip D.,
Bynum James A,
Klemcke Harold
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1155.16
Subject(s) - gene expression , gene , andrology , biology , significance analysis of microarrays , rna , strain (injury) , microbiology and biotechnology , survival analysis , dna microarray , immunology , genetics , medicine , anatomy
A marked differential survival (0.5 to 6 h) time of multiple rat strains to severe hemorrhage was previously demonstrated (Klemcke et al; Shock, in press 2007). To explore a genetic contribution to differential outcomes, gene expression analysis was performed on long surviving Brown Norway/Mcwi (BN; ∼300 min) and short surviving (DA/OlaHsd (DA;∼40 min)) inbred strains. Anesthetized rats (5 rats per group) were catheterized and, ∼ 24 hours later, 55% of the calculated blood volume was removed during a 26 min period from conscious unrestrained animals. The first ml of blood and the final ml were taken for RNA isolation. RNA was labeled with Cy3 or Cy 5 and hybridized to Agilent rat whole genome microarrays and analyzed for statistical differences in gene expression. No differences in expression were noted between start and finish of hemorrhage. Between strain comparisons of expression patterns indicated large difference between the long surviving BN and short‐surviving DA. 1056 genes were statistically different in their expression with a false discovery rate <5%. Analysis of statistically altered expression patterns with Ingenuity Pathway Analysis™ program indicated differences between long and short surviving strains including pathways for free radical scavenging, organismal survival and mitochondrial dysfunction that may explain differences in survival times.