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Neurogenic control of cerebral vasculature: potential role of P450 Epoxygenase
Author(s) -
Iliff Jeffrey J.,
Wang Ruikang,
Baumann Thomas K,
Alkayed Nabil J
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1151.23
Subject(s) - vasodilation , epoxide hydrolase 2 , stimulation , cerebral circulation , capsaicin , endocrinology , cerebral arteries , medicine , epoxygenase , middle cerebral artery , gene isoform , sensory stimulation therapy , chemistry , anatomy , cytochrome p450 , biochemistry , enzyme , metabolism , ischemia , gene , receptor
Epoxyeicosatrieonic acids (EETs) are cerebral vasodilators formed by P450 epoxygenases (EPOX) and metabolized by soluble epoxide hydrolase (sEH). We recently reported the presence of EPOX and sEH in vasodilator nerves surrounding large cerebral arteries. In the current study, we elicited dilation of these vessels by trigeminal ganglia (TG) sensory stimulation and examined the expression of EPOX and sEH in TG. Western blot detected sEH expression in rat and mouse TG (n=2 each), and RT‐PCR identified P450 2J but not 2C EPOX isoforms in rat (n=2 from 8 animals) and mouse TG (n=1 from 3 animals). Intra‐oral sensory stimulation with capsaicin (50μl, 1–100μM) evoked vasodilation in the mouse MCA territory (14% maximal response, n=2), detected by optical micro‐angiography. These findings demonstrate that intra‐oral sensory stimulation elicits cerebral artery dilation, and that TG neurons express components of the EETs signaling system. Future studies will evaluate if EETs mediate the hyperemic response to capsaicin, and the neuroanatomic basis for such response. Supported by NS44313 to NJA and a predoctoral NRSA to JJI.