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Accuracy of mouse aortic diameter measurement: comparison of videomicrometer and ultrasound
Author(s) -
xiong Wanfen,
Knispel rebecca A.,
baxter bernard timothy
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1148.4
Subject(s) - ultrasound , medicine , aneurysm , abdominal aorta , abdominal aortic aneurysm , aorta , linear correlation , aortic aneurysm , ultrasonography , nuclear medicine , radiology , surgery , mathematics , statistics
Objective: The purpose of this study was to determine the accuracy of high‐resolution ultrasound in measuring the diameter of infrarenal abdominal aorta and to compare its use to videomicrometer, which is the most commonly used method in the studies of animal models of abdominal aortic aneurysm (AAA). Methods: Mice (C57BL/6), at 8 weeks age, underwent AAA induction by periaortic application of CaCl 2 . The diameter of the infrarenal aorta was measured at aneurysm induction and 8 weeks after aneurysm induction with videomicrometer and ultrasound by M‐mode ultrasonography. Results: The mean diameter of the infrarenal abdominal aorta at aneurysm induction was 535 ± 8.3 μm by videomicrometer and 531 ± 7.9 μm by ultrasound. Eight weeks after CaCl 2 aneurysm induction, the mean aortic diameter was 892 ± 59.1μm by videomicrometer and 902 ± 55.8 μm by ultrasound. The mean differences of the measured diameters by the two methods were 7.8 μm (SE=12.2 μm) at initial measurement and 10.3 μm (SE=36.9 μm) at 8 weeks after aneurysm induction. The linear correlation coefficient ( r value) for the diameter after 8 weeks aneurysm induction was 0.785. Using this criteria, there was a consistent, relative narrowing calculated by both videomicrometer and ultrasound. Conclusion: The mean difference, when taken together with a good linear correlation coefficient of the diameters as measured by videomicrometer and ultrasound, makes this technique acceptable. In addition, ultrasound is suitable for keeping track of the aortic diameter changes during mouse aneurysm development and treatment without open surgery.