Premium
Sexual and Maturational Difference in Phosphodiesterase mRNA Expression in Rat Skeletal Muscle Microvascular Endothelial Cells
Author(s) -
Wang Jianjie,
Huxley Virginia H.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1145.4
Subject(s) - phosphodiesterase , phosphodiesterase 3 , medicine , endocrinology , microvessel , basal (medicine) , intracellular , messenger rna , skeletal muscle , biology , chemistry , enzyme , angiogenesis , microbiology and biotechnology , gene , biochemistry , insulin
The levels of intracellular cAMP and cGMP, important mediators of microvessel permeability ( P s ), are regulated predominantly by the rates of synthesis and hydrolysis. Phosphodiesterases (PDE) are central to the stimulation or inhibition of hydrolysis of cAMP and cGMP. Our previous studies demonstrated that basal P s to albumin was greater in adult males (AM) than adult females (AF), juvenile males (JM) and females (JF), but there were no differences among AF, JM, and JF. Therefore, we hypothesized differential PDE expression in AM relative to AF, JM, and JF, resulting in different levels of cAMP and cGMP and consequent difference in basal P s . In this study we used endothelial cells (EC) derived from rat skeletal muscle microvessels of AM, AF, JM and JF used for P s studies to detect PDE 1–5 mRNA expression via real time RT‐PCR analysis. We found that mRNA level of PDE1A was 2.5‐fold higher in JM than JF ( n =5). PDE2A expression was dependent on maturity while PDE2A levels were higher in juveniles than adults (n=7) in both sexes, and 2‐fold higher in JF (n=6) than JM (n=7). PDE3B was expressed 2.4‐fold higher in JM (n=7) than AM (n=7). PDE4D expression did not differ among the 4 groups of EC. JM (n=5) expressed higher PDE5A than AM (n=6). The findings indicate differential expression of PDE isoforms among the 4 groups of microvessel EC. However, the relationship between PDE expression and levels of cAMP and cGMP remains to be elucidated.