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Perfusate composition is a determinant of the hydrodynamically relevant endothelial glycocalyx thickness in vivo.
Author(s) -
Potter Daniel Raymond,
Jiang John,
Damiano Edward
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1143.6
Subject(s) - glycocalyx , in vivo , cremaster muscle , biophysics , arteriole , chemistry , pipette , microcirculation , lumen (anatomy) , anatomy , biomedical engineering , microbiology and biotechnology , biology , biochemistry , medicine
The endothelial‐cell glycocalyx surface layer is a highly hydrated structure that is constantly bathed in the perfusing fluid, typically blood in vivo. It is proposed that there is a constant exchange of molecules between the blood and the endothelial glycocalyx that is essential to the integrity of the layer. To test the effect of different prefusate compositions on the endothelial glycocalyx in vivo, a micromanipulator is used to introduce a micropipette into the lumen of one of the primary feed arterioles in a mouse cremaster‐muscle preparation. The micropipette then steadily perfuses the microvascular network fed by that arteriole with either native plasma, cell‐culture media containing 20% serum, or saline. Micro‐particle image velocimetry data is then collected from downstream microvessels. Velocity profiles extracted from the μ‐PIV data are then used to estimate the effective hydrodynamically relevant thickness of the glycocalyx. Preliminary data indicate that vessels perfused with saline have no detectable hydrodynamically relevant layer. Supported by NIH grant R01 HL76499.