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Role of adenosine A2a receptor in regulation of cerebral blood flow (CBF) during induced hypotension
Author(s) -
Kusano Yoshikazu,
Miekisiak Grzegorz,
Kulik Tobias,
Sandler Adam,
Winn Richard H.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1143.3
Subject(s) - adenosine a2a receptor , adenosine , cerebral blood flow , medicine , antagonist , hemodynamics , anesthesia , receptor antagonist , blood pressure , receptor , blood flow , endocrinology , adenosine receptor , chemistry , agonist
The aim of this study is to evaluate the role for adenosine A2a receptor during induced hypotension in mice. Methods: Anesthetized and ventilated wild type (WT) and adenosine A2a receptor knockout (KO) mice (C57Bl/6) were used. Catheters were placed in femoral arteries for measurement of mean arterial pressure (MABP) and withdraw the arterial blood. MABP was lowered to 35 mmHg by continuously withdrawing arterial blood at a rate of 25 μl/min. CBF was measured using the laser‐Doppler flowmetery through the intact calvarium. We also studied the effects of specific A2a receptor antagonist, ZM‐241385 (1 mg/Kg and 5 mg/Kg, ip). Results: During hypotension CBF was attenuated by 38.9% in WT (n=5) and 51.1% in KO mice (n=7) (P<0.05). The low dose of ZM‐241385 (1 mg/Kg) (n=6) had no effect on reduction of CBF. However with a higher dose (5 mg/Kg) (n=6), the CBF significantly reduced by 51.3% (P<0.05). Conclusions: These results suggest that A2a receptor is involved in the attenuation of CBF during induced hypotension. Supported by NIH‐NS 21076 (HRW)