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Using Tyrosine 654 Phosphorylation of Beta‐Catenin to Predict HCC Pathology
Author(s) -
Cieply Benjamin Walter,
ProverbsSingh Tracey,
Kaur Manpreet,
Monga Satdarshan
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1124.7
Subject(s) - hepatocellular carcinoma , phosphorylation , cirrhosis , tyrosine , medicine , tyrosine phosphorylation , exon , beta catenin , gastroenterology , pathology , blot , liver cancer , cancer research , biology , gene , wnt signaling pathway , receptor , biochemistry
Background: Our aim was to study what roles β‐Catenin (β‐Cat) and tyrosine 654 phosphorylation of β‐Cat were playing in liver cancer (HCC). Design: Protein from 28 HCC cases and 3 cases without HCC (controls) was analyzed by western blots. Results: Of the 28 samples, 1 was poorly (P), 20 were moderately (M) and 5 were well (W) differentiated with 2 of unknown (U) status. 5/28 were fibrolamellar (FL). Only P or M tumors showed invasion (I). Of the non‐cirrhotic samples, I HCC had higher levels of tyr654 compared to non‐I. Of non‐cirrhotics and non‐FL HCC, M HCC had higher levels of tyr654 than W. Interestingly, W had higher levels of total β‐Cat. FL HCC had highest levels of tyr654 but lowest β‐Cat. HCC w/mutations at exon 3 had high tyr654 and high total β‐Cat. Conclusions: Tyr654 may be useful to differentiate M from W HCC and I from non‐I HCC. Also, FL HCC may have increased levels of tyrosine kinases inducing the phosphorylation of β‐Cat at tyr654. We found no correlation between β‐Cat mutations and differentiation, invasion or cirrhosis status.