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Connecting the Genome to the Cytoplasm
Author(s) -
Silver Pamela
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.112.1
Subject(s) - nucleoplasm , cytoplasm , microbiology and biotechnology , biology , ribosome , rna , translation (biology) , nuclear export signal , rna binding protein , messenger rna , nucleolus , genome , nuclear transport , cell nucleus , genetics , gene
The movement of mRNAs through the nucleoplasm and out of the nucleus to cytoplasmic locations is an elaborately orchestrated and regulated process. The pathway of mRNA export includes: proper processing, packaging into protein‐RNA complexes, targeting and movement through the nuclear pore complex and release into the cytoplasm for translation. In some cases, mRNAs are further localized within the cytoplasm. The presentation will focus on the spatial organization of the genome with respect to its interactions with the nuclear pore complex, nuclear export factors, RNA binding proteins, and the processing and movement of mRNAs out of the nucleus. We have taken a systems‐wide approach to delineating the complex relationship between mRNA production, processing and transport. In particular, we have used genome‐wide analysis to determine the differential interactions between proteins involved in all stages of the process and the entire genome of both yeast and human cells. In doing so, we have identified the action of new factors in metazoan cells and made the surprising finding that there are distinct ribosomes with specialized functions.