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Identification of genes associated with neural development in puppies as indicated by MRI
Author(s) -
Frantz Nolan Z.,
Zicker Steve C.,
Yamka Ryan M.,
Friesen Kim G.,
Gao Xiangming,
AlMurrani Samer
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1117.6
Subject(s) - grey matter , white matter , biology , gene , dry matter , rna , zoology , magnetic resonance imaging , medicine , genetics , radiology
Microscopic resonance imaging (MRI) was performed on 78 (Age = 1 year) beagle puppies as a non‐invasive means to determine relative amounts of grey and white brain matter. The fifteen puppies with the greatest amount of total grey and white matter were compared to the fifteen puppies with the least amount of total grey and white matter from the original 78. Puppies were fed commercially available foods for one year and then a blood sample was drawn into a PAXgene Blood RNA tube. RNA was extracted according to the procedures provided in the PAXgene Blood RNA Kit Handbook (Qiagen, Valencia, CA). RNA was hybridized to an Affymetrix GeneChip Canine‐2 Genome Arrays and normalized using Robust Multi‐Array Average. Transcripts having a P < 0.01 (following a false discovery rate adjustment value of 0.1) and a fold change range of at least 1.3 were considered different among the two groups. Analysis of the RNA found differences in 107 genes between the fifteen puppies with greatest amount of grey and white matter and puppies with least amount of grey and white matter. Of the genes identified, 43 were up‐regulated and 64 down‐regulated in puppies with the greatest amount of grey and white matter when compared to puppies with least grey and white matter. Genes associated with growth hormone signaling and neuron development were increased, while inflammation and fatty acid biosynthesis were decreased in puppies with greatest amount of grey and white matter compared to puppies with the least amount of grey and white matter.