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Tissue Expression of Fasting‐Induced Adipocyte Factor in Cattle
Author(s) -
Mamedova Liaman,
Bradford Barry,
Robbins Kabel,
Johson Brad
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1116.2
Subject(s) - abomasum , omasum , adipose tissue , duodenum , ileum , rumen , gastrointestinal tract , biology , medicine , western blot , jejunum , small intestine , adipocyte , endocrinology , biochemistry , gene , fermentation
Fasting‐induced adipocyte factor (FIAF; also known as angiopoietin‐like 4) is a plasma protein that stimulates the oxidation of fatty acids and inhibits fat accumulation. The gastrointestinal tract appears to play an important role in regulating plasma FIAF concentration in some situations. Bovine FIAF shares 87% homology with the mouse protein. Our aim was to determine tissue expression of FAIF in the bovine. Rumen, omasum, abomasum, duodenum, jejunum, ileum, colon, pancreas, liver and adipose tissue samples were collected postmortem from 2 steers. FIAF mRNA was quantified by qPCR, and was most abundant in liver (420 arbitrary units; AU) and adipose tissue (320 AU). We detected FIAF mRNA throughout the gastrointestinal tract, with values ranging from 10 AU in the abomasum to 28 AU in the abomasum. By Western blot, FIAF protein was most abundant in liver and adipose tissue. However, FIAF protein was barely detectable in the rumen or duodenum, but was highly abundant in the omasum. Finally, cross‐sections of the rumen wall were used for immunohistochemistry staining, and strong signals for FIAF were detected in the epithelial layer. These findings suggest host/microbe interactions may influence FIAF expression in the ruminant gastrointestinal tract.