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Effect of Pycnogenol on Glucose Transporter4 expression and glucose uptake stimulatory activity in 3T3‐L1 adipocytes
Author(s) -
Lee HeeHyun,
Kim KuiJin,
Yoon KyeYoon,
Lee BooYong
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1113.1
Subject(s) - glucose uptake , chemistry , glucose transporter , wortmannin , 3t3 l1 , pharmacology , mapk/erk pathway , insulin , catechin , biochemistry , quercetin , kinase , adipocyte , antioxidant , endocrinology , biology , adipose tissue , phosphatidylinositol , polyphenol
Pycnogenol, a standardized extract from the bark of the French maritime pine, consists of phenolic compounds including catechin, taxifolin, procyanidins, and phenolic acids. Procyanidins of pycnogenol are flavonoids with an oligomeric structure, and it has been shown that they can improve the pathological oxidative state of a diabetic situation. To evaluate whether the effects of pycnogenol were due to their action on insulin‐sensitive tissues, we used the insulin‐sensitive cell line 3T3‐L1 adipocytes. And then we used the glucose uptake assay and western blotting to see Gult4 movement. Pycnogenol stimulated glucose uptake in 3T3‐L1 adipocytes by a dose‐dependent manner. Pycnogenol significantly stimulated glucose uptake in 3T3‐L1 adipocytes at the concentration of 250ug/mL. To determine whether PI3K and p38 MAPK were involved in the signaling pathways by Pycongenol, we performed assays using specific inhibitors of these kinases. As a result, the effect of Pycnogenol on glucose uptake was sensitive to wortmannin, an inhibitor of phosphoinositol 3‐kinase and to SB203580, an inhibitor of p38MAPK. Conclusively Pycnogenol have insulin‐like effects in insulin sensitive cells that could help to explain their anti‐hyperglycemic effect in vivo. These results suggest that Pycnogenol may be useful for the prevention and treatment of diabetes.