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Structure‐Activity Relationships of Components from the Roots of Pueraria thunbergiana Having Aldose Reductase Inhibitory and Antioxidative Activity
Author(s) -
Jeon Young Eun,
Kang IlJun,
Park ChangHun,
Lim Soon Sung,
Lee DongUng
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1111.2
Subject(s) - aldose reductase , pueraria , isoflavonoid , daidzin , chemistry , daidzein , genistin , puerarin , naringin , hesperidin , flavonoid , genistein , isoflavones , polyol pathway , hesperetin , baicalein , formononetin , antioxidant , biochemistry , traditional medicine , pharmacology , enzyme , chromatography , medicine , alternative medicine , pathology
Aldose reductase (AR), the key enzyme of the polyol pathway, has been demonstrated to play an important role in the etiology of diabetic complications; therefore, AR inhibitors would be expected to be effective in preventing cataract formation in diabetes mellitus. The 80% methanol extract and fractions of the roots of Pueraria thunbergiana were tested for their inhibitory activities on rat lens AR in order to find the most active fraction. For activity‐guided separation, we isolated four isoflavonoids, daidzin, daidzein, puerarin, and ononin, and identified their structures by comparison of their spectral data with previously reported data. The effects of the constituents of Pueraria root, including the above compounds 3–6, compound 7 (formononetin) prepared from compound 3 and the commercial isoflavonoids, genistin and genistein, and flavonoids, baicalin and baicalein, were estimated with rat lens AR using DL‐glyceraldehyde as a substrate. And also, antioxidative activities of the fractions and the constituents were investigated. Considering the close association between AR inhibitor and antioxidant, isoflavonoid 4 and flavonoid 9 would be acceptable compounds for which further trials including in vivo testing should be conducted. Supported by Brain Korea 21.