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Puerarin and its glycosides do not show toxicity in vitro and in vivo.
Author(s) -
Chung Hyuk jin,
Chung Mi ja,
Ham SeungShi,
Park Kwan Hwa,
Lee Sung joon
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1108.9
Subject(s) - puerarin , glycoside , pharmacology , in vivo , chemistry , genotoxicity , micronucleus test , toxicity , micronucleus , biochemistry , biology , medicine , stereochemistry , organic chemistry , alternative medicine , microbiology and biotechnology , pathology
Puerarin is an isoflavone derived from Kudzu roots and has antioxidant and hypocholesterolemic effects; however, its insolubility often limits its biological availability in vivo. Using a novel transglycosylation process, the solubility of puerarin glycosides was increased >100‐fold. High levels of puerarin and its glycosides might exert toxicity due to an estrogenic activity and other biological effects, thus, we investigated potential toxicity of puerarin and its glycosides. Mutagenic effects were investigated with Ames test and the results showed that both puerarin and its glycosides did not have dose‐dependent mutagenic effects. Antimutagenic properties of puerarin and its glycosides were tested with 4‐nitro‐quinoline‐1‐oxide as a mutagen. The results showed that the puerarin and its glycosides were able to significantly inhibit the mutations up to 50% of the controls. In addition, in vivo genotoxicity and cytotoxicity were examined by bone marrow micronucleus assay with ICR mice after oral administration of puerarin and its glycosides. Both puerarin and its glycosides did not reveal significant genotoxic and cytotoxic properties. Finally, Sprauge‐Dawley rats were orally administered with puerarin and its glycosides to investigate subchronic safety for 2 months. It was found that the administration of both puerarin and its glycosides did not significantly affect plasma and histological toxicity markers. These results suggest that puerarin and its glycosides do not have significant toxic effects both in vitro and in vivo , and may be safe as functional foods or other health related industrial applications.