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Oral supplementation of gromwell (Lithospermum erythrorhizon) extract prevents the development of atopic dermatitis with reducing ceramide degradation in the epidermis of NC/Nga mice
Author(s) -
Kim JungMin,
Kim YoungRan,
Seo DaeBang,
Kim SungHan,
Lee SangJun,
Cho Yunhi
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1108.8
Subject(s) - ceramide , atopic dermatitis , epidermis (zoology) , transepidermal water loss , sphingolipid , chemistry , enzyme , immunoglobulin e , endocrinology , medicine , biochemistry , immunology , antibody , stratum corneum , pathology , apoptosis , anatomy
Gromwell ( Lithospermum erythrorhizon ) is widely used in the treatment of abnormal skin condition, but its systemic efficacy, especially in atopic dermatitis (AD), is not clear. To examine the systemic efficacy of gromwell on the clinical manifestation of AD‐like skin lesions, NC/Nga mice, a murine model of AD, were fed control diet (group CA: atopic control) or diet with 70% ethanol extracts from 5% gromwell (Group LE) for 10 wks. In group LE, the clinical manifestation of AD‐like skin lesion was prevented as the level of serum IgE, epidermal hyperproliferation, and the number and duration of scratching episode, which were greater in group CA, were significantly reduced to the similar level of normal control group of BALB/c mice. In addition, the level of ceramides, the major lipid maintaining epidermal barrier, in the epidermis of group LE was increased, which was inversely associated with a decreased protein level of ceramidase, an enzyme of ceramide degradation. However, mRNA and protein levels of serine palmitoyl transferase (enzyme for de novo ceramide synthesis) in groups C, CA and LE did not differ. We demonstrate that oral supplementation of gromwell extracts prevents the development of atopic dermatitis with reducing ceramide degradation coupled with a low expression of ceramidase protein.