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Inhibition of chlorophyll derivatives on aflatoxin B1‐induced damage in hepatoma cells
Author(s) -
HSU CHINGYUN,
HU SHENEPIN,
CHEN CHIAOMING
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1096.6
Subject(s) - aflatoxin , chemistry , glutathione , pheophorbide a , biochemistry , dna damage , enzyme , microbiology and biotechnology , chlorophyll , dna , biology , food science , organic chemistry
The inhibitory effects of four chlorophyll derivatives (chlorophyllide a and b [Chlide a and b] and pheophorbide a and b [Pho a and b]) on aflatoxin B 1 (AFB 1 )‐DNA adduct formation, and on the modulation of hepatic glutathione S‐transferase [GST] in murine hepatoma (Hepa‐1) cells were evaluated. Enzyme‐linked immunosorbent assay was used for the former and spectrophotometric assay for the latter. Pretreatment with Chlide or Pho (20 and 50 μM) significantly reduced the formation of AFB 1 ‐ DNA adducts, and Pho was the more potent inhibitor. However, wash out prior to adding AFB 1 totally eliminated inhibition by Childe and partially eliminated inhibition by Pho, indicating the inhibitory effect of Chlide, and to some extent Pho, was mediated through direct trapping of AFB 1 . Furthermore, Pho or Chllin treatment can also increase GST activity in Hepa‐1 cell. These observations indicate that all the chlorophyll derivatives may attenuate AFB 1 ‐induced DNA damage in the Hepa‐1 cell by direct trapping of AFB 1 . Pho had more protective effect not only by direct trapping but also modulating GST activity to hepatic AFB 1 metabolites.