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Basal and amino acid stimulated fractional synthesis rates of calf muscle protein subfractions in peripheral arterial disease
Author(s) -
Tuvdendorj Demidmaa,
Wolfe Robert R,
Killewich Lois A
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1095.4
Subject(s) - myofibril , basal (medicine) , phenylalanine , medicine , sarcoplasm , peripheral , endocrinology , chemistry , amino acid , contraction (grammar) , biochemistry , endoplasmic reticulum , diabetes mellitus
Basal fractional synthesis rate (FSR) and the effect of essential amino acid (EAA) supplementation on protein synthesis of specific subfractions of calf muscle in peripheral arterial disease (PAD) is presented. Six patients with PAD and 6 healthy, age‐ and sex‐matched controls were studied. FSR was measured in the basal (post‐absorptive, PA) state and following administration of a single oral bolus of 15g of EAA. Sarcoplasmic, mitochondrial and myofibrillar protein FSRs were calculated from gas chromatography/mass spectroscopy measurements of the incorporation of the stable isotope L‐[ring‐ 2 H 5 ]‐phenylalanine into muscle proteins. Data are expressed as mean±SE; statistical analyses were performed using two‐way repeated ANOVA. Results: In both groups, in comparison with PA, EAA stimulated mitochondrial (PAOD: 0.055±0.011 vs 0.110±0.017 %/h; control: 0.063±0.019 vs 0.130±0.027 %/h; PA vs EAA, P<0.05) and sarcoplasmic (PAOD: 0.065±0.011 vs 0.123±0.008 %/h; control: 0.075±0.012 vs 0.110±0.013 %/hr; P < 0.05) protein FSR, but not myofibrillar (PAOD: 0.070±0.020 vs 0.100±0.011 %/h; control: 0.070±0.022 vs 0.070±0.017 %/h; P= NS). Conclusion: Basal and EAA stimulated protein FSR subfractions of gastrocnemius muscle are not different in PAD compared to healthy control subjects.