Premium
Effects of Marginal Vitamin B6 Deficiency on Plasma Fatty Acid Profiles in Healthy Men and Women
Author(s) -
Zhao Mei,
Lamers Yvonne,
Stacpoole Peter W,
Gregory Jesse F
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1092.9
Subject(s) - arachidonic acid , medicine , chemistry , endocrinology , linoleic acid , fatty acid , vitamin d deficiency , vitamin , metabolism , vitamin d and neurology , biochemistry , enzyme
The interaction between vitamin B 6 (VB 6 ) and lipid metabolism is not well understood. Previous studies indicated that linoleic acid (LA) levels increased and arachidonic acid (AA) levels decreased in different tissues of VB 6 ‐deficient rats, suggesting an altered n‐6 pathway of fatty acid (FA) synthesis. Our study aimed to determine the effect of marginal VB 6 deficiency on plasma FA in human subjects. Thirteen healthy subjects (7 men and 6 women, aged 20–39 yr) received a 2‐d adequate diet followed by a 28‐d VB 6 restricted diet (0.5 mg/d) to reach a marginal VB 6 deficiency (plasma pyridoxal‐5′‐phosphate 20–30 nM). Fasting blood samples were collected after 2‐d of adequate diet and after 28‐d of VB 6 restricted diet. Plasma total FA profiles were analyzed by gas chromatography/flame ionization detection. After 28‐d of VB 6 restriction, AA concentration decreased significantly from (mean ± SD) 533 ± 72 nM to 479 ± 59 nM (p=0.004). The conversion indices of AA/LA also significantly decreased from 0.23 ± 0.04 to 0.20 ± 0.04 (p<0.001). There was a slight but non‐significant increase in plasma LA concentration. However, we did not observe any significant alteration in n‐3 fatty acid concentrations. In conclusion, marginal VB 6 deficiency is associated with alterations in the distribution of n‐6 fatty acids in plasma lipids in healthy men and women. Supported by NIH grant DK072398 and GCRC grant M01‐RR00082.