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Dietary n‐3 fatty acids alter lipid profile in allograft heart tissues in rats
Author(s) -
Suh Miyoung,
Van Betty TT,
Othman Rgia A,
Moghadasian Mohammed H
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1091.3
Subject(s) - fish oil , polyunsaturated fatty acid , transplantation , lipid metabolism , heart transplantation , fatty acid , endocrinology , medicine , lipid profile , chemistry , biology , biochemistry , fish <actinopterygii> , cholesterol , fishery
This study investigated the influence of dietary n‐3 polyunsaturated fatty acids on lipid metabolism in native and allograft hearts. Cardiac allografts were produced through abdominal heterotopic cardiac transplantation in rats by using male Fischer and Lewis rats being donors and recipients, respectively. After surgery animals were randomly assigned into one of the 3 groups fed a PicoLab rat chow supplemented with safflower oil (control, 5% w/w), flaxseed oil (5% w/w) or fish oil (2%, w/w) and received cyclosporine (1.5 mg/kg/d). Native and graft hearts were collected for lipid analysis after 12 weeks. Graft hearts showed significant increases in the levels of TG (p<0.0001) and MG (p<0.05), but decrease in FFA, Chol, and PL in comparison to native hearts. The increase of TG and MG was much higher in graft hearts from rats fed the fish oil diet. The incorporation of dietary PUFA into PL was significantly lower in graft hearts compared to native hearts. Animals fed fish oil diet significantly increased 22:6n‐3 levels, in both native and graft heart in PL in comparison to rats fed the control and flax diet. These results show that abnormal lipid metabolism occurs in heart after heart transplantation. Dietary n‐3 fatty acids influence the lipid profile and fatty acid compositions in graft hearts implying therapeutic strategies for post‐transplantation care. (supported by NSERC & HSF).