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Interaction between dietary fructose and protein: marginal protein deficiency induces pyruvate dehydrogenase kinase 4 (PDK4) and glucose transporter 4 (GLUT4) genes in rat muscle
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1088.5
Subject(s) - glut4 , medicine , endocrinology , pdk4 , glucose transporter , fructose , pyruvate kinase , chemistry , glycolysis , glut1 , glucose uptake , insulin , carbohydrate metabolism , metabolism , pyruvate dehydrogenase complex , biology , biochemistry , enzyme
In animal models, marginally protein deficient diets increase adiposity, while high fructose diets cause hyperlipidemia and hypertension. The purpose of this study was to evaluate the interactive effects of dietary protein and fructose on metabolic syndrome. Male Sprague‐Dawley rats (n=24) were fed one of 6 diets varying in macronutrients for 30 days. Diets were based on a 2x3 factorial with 2 levels of protein (10% (P10) and 20% of energy), and 3 levels of fructose (0% (F0), 20%, and 40% of energy, replacing glucose). Baseline and final body composition were measured by dual energy X‐ray absorptiometry. Final blood pressure was measured by tail cuff. While fructose tended to increase blood pressure (p=0.06), it was P10‐F0 that had the highest percent body fat (p<0.005) and cumulative food intake (p<0.05). In P10 compared to P20 groups, lean body mass was lower (p=0.008), while muscle PDK4 mRNA was higher (p=0.04), suggesting decreased muscle protein synthesis accompanied by decreased glucose oxidation. Unexpectedly, muscle GLUT4 mRNA was higher in P10 groups (p=0.01), whereas there was no difference in plasma insulin between any groups. In conclusion, 1) no significant interaction between dietary fructose and protein was observed, and 2) marginal protein deficiency may shift glucose metabolism toward anaerobic glycolysis.