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Characterization of The Bovine Mammary Serotonergic System
Author(s) -
Hernandez Laura Lorraine,
Limesand Sean W.,
Baumgard Lance H,
Horseman Nelson D,
Collier Robert J
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1080.1
Subject(s) - serotonin , receptor , 5 ht receptor , serotonergic , chemistry , in situ hybridization , tryptophan hydroxylase , serotonin transporter , microbiology and biotechnology , mammary gland , endocrinology , medicine , biology , biochemistry , gene expression , gene , cancer , breast cancer
Previous research indicates serotonin ( 5‐HT ) as a feedback inhibitor of lactation in the bovine. We utilized quantitative real‐time PCR ( qRT‐PCR ) and in situ hybridization of tissue cryosections to identify 5‐HT receptors in bovine brain ( BB ), mammary tissue ( BMT ) and primary bovine mammary epithelial cells ( BMEC ). Additionally, we utilized immunohistochemistry in BB and BMT cryosections to determine presence of tryptophan hydroxlase 1 ( TPH‐1 ), aromatic amino acid decarboxylase ( AADC ), and the serotonin reuptake transporter ( 5HTT ). Serotonin receptors 1b, 2a, 2b, 4, and 7 were detected in the BB, BMT and BMEC by PCR and qRT‐PCR. In BMT, fold expression of receptors relative to BB, (highest to lowest) was: 5‐HT 1b (2,477; P < 0.0001), 5‐HT 4 (389), 5‐HT 2b (89), 5‐HT 7 (18), and 5‐HT 2a (3.77). In BMEC, the fold expression of receptors relative to BB (highest to lowest) was: 5‐HT 4 (230, P < 0.0001), 5‐HT 2b (44), 5‐HT 7 (37), 5‐HT 1b (30) and 2a (1.64). Serotonin receptors 1a, 1d, 1f, and 2c were only detected in the BB by PCR. Immunostains for TPH‐1, AADC, and 5HTT were positive in both BB and BMT. We conclude that BMT and BMT enriched with BMEC contain the 5‐HT 1b, 2a, 2b, 4 and 7 receptors. The enrichment of tissue preparation with BMEC had the highest expression of the 5‐HT 4 receptors relative to BMT, while BMT had the highest expression of 5‐HT 1b receptors relative to BMEC. Manipulation of 5‐HT receptors with specific antagonists present in BMEC could lead to increased milk protein synthesis. *Funded by USDA‐NRI#2007‐35206‐17898