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In vivo evaluation of the immunomodulatory activity of the polysaccharides purified from Salicornia herbacea
Author(s) -
Im SunA,
Lee YoungRan,
Lee YoungHee,
Kim Hyunjin,
Chang PanYoung,
Jung KiSung,
Lee ChongKil
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1078.16
Subject(s) - polysaccharide , in vivo , immune system , in vitro , chemistry , ovalbumin , nitric oxide , biology , cell culture , biochemistry , immunology , genetics , microbiology and biotechnology , organic chemistry
Immunomodulatory polysaccharides isolated from plants or mushrooms have been shown to activate diverse types of cells of the immune system in vitro . However, the in vivo efficacy of the immunomodulation has been a matter of question due to the high molecular weight nature of the polysaccharides. In the present study, we examined the in vivo immunomodulatory activity of the polysaccharides isolated from Salicornia herbacea . The immunomodulatory polysaccharides were purified from the hot water extract of Salicornia herbacea by passage through a DEAE‐Sephacel column, and then the gel filtration chromatography. The polysaccharides efficiently induced cytokine production, nitric oxide release, expression of surface molecules and phagocytic activity, in a dose dependent manner, when added to cultures of a mouse monocytic cell line, RAW 264.7 cells. In vivo efficacy was examined in mice implanted with tumor cells, and also in mice immunized with ovalbumin (OVA). We found that the polysaccharides enhance antitumor immune responses in mice implanted with Sarcoma‐180 cells. In addition, we found that the polysaccharides induce strong OVA‐specific CTL responses in mice. These results confirm that the polysaccharide purified from Salicornia herbacea exerts immunostimulatory activity in vivo .