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IgE accelerates in vitro development and modifies the phenotype of mast cells
Author(s) -
Kashiwakura Junichi,
Xiao Wenbin,
Kitaura Jiro,
Kawakami Yuko,
Wilson Bridget S.,
Blank Ulrich,
Kawakami Toshiaki
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1075.13
Subject(s) - degranulation , mast cell , immunoglobulin e , stem cell factor , microbiology and biotechnology , tryptase , cytokine , interleukin 33 , cell , immunology , chemistry , in vitro , progenitor cell , biology , antibody , stem cell , biochemistry , interleukin , receptor
Monomeric IgEs possess a wide range of heterogeneity in their ability to induce mast cell activation in the absence of multivalent antigen. Highly cytokinergic (HC) IgEs induce a variety of mast cell activation events including cell survival, degranulation, cytokine production, and migration, whereas poorly cytokinergic (PC) IgEs do so inefficiently. Here we investigated the effect of monomeric IgE molecules on mast cell differentiation. When bone marrow cells are cultured with monomeric IgEs, cell number is increased compared to cultures without IgE. Furthermore, time in culture required to generate greater than 80% pure mast cells is decreased. IgEs can induce mast cell progenitors to differentiate into mast cells. mRNA expression levels of several mast cell proteases and mast cell‐related transcription factors such as Egr‐1, a transcription factor involved in the production of TNF‐α, are increased in mast cells cultured with an HC IgE compared to those cultured with a PC IgE or without IgE. Consistent with this, expression of TNF‐α is higher in mast cells cultured with an HC IgE. Taken together, these results suggest that monomeric IgEs, especially HC IgEs, not only promote mast cell development, but also modulate the mast cell phenotype. Funded in part by the NIH grant AI50209.