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Expression of TNF‐α, IFN‐γ, TGF‐β and IL‐7 on cells isolated from the Bronchoalveolar Fluid in a model of secondary immunodeficiency.
Author(s) -
Luczak Erica Natalia,
Calabrese Graciela Cristina,
Roux Maria Estela
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1072.12
Subject(s) - bronchoalveolar lavage , cytokine , immunology , tumor necrosis factor alpha , transforming growth factor , western blot , biology , flow cytometry , microbiology and biotechnology , medicine , lung , endocrinology , gene , biochemistry
We have previously reported the early appearance (4 days post partum) of γδT cells that colocalize with TNF‐α in Bronchus‐Associated Lymphoid Tissue (BALT), indicating the important role of this cytokine for BALT development. In our experimental model of secondary immunodeficiency due to severe protein deprivation at weaning followed by administration of a 20% casein diet during 21 days (R21), we also demonstrated an increase of TCR γδ+ cells accompanied by an increase in TNF‐α+ cells number in R21. The objective of this work is to analyze and compare cells isolated from the bronchoalveolar fluid (BAL) in R21 vs control rats: 1) the expression of TNF‐α and IFN‐γ, as indicator of an inflammatory process and its regulation by TGF‐β and 2) the cellular regulation by IL‐7. Western Blot analysis showed an increase of all cytokines in cells from BAL of R21 animals when compared to control rats (Figure 1). Our results indicate the existence of an inflammatory process (due to TNF‐α and IFN‐γ) modulated by TGF‐β. The increment of IL‐7 correlates with its capacity for the proliferation and differentiation of γδ T cells, which are increased in BAL from R21 as previously reported. Moreover, we suggest a protective role of IL‐7 in the inflammatory focus. Supported by B010 grant.