Premium
Differential gene expression with co‐engagement of FcεRI and CC chemokine receptor 1
Author(s) -
Aye Cho Cho,
Morohoshi Kei,
Fukuda Ken,
Fifadara Nimita,
Toda Masako,
Ono Santa Jeremy
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1070.29
Subject(s) - degranulation , ccr1 , c c chemokine receptor type 6 , chemokine receptor , cc chemokine receptors , microbiology and biotechnology , chemokine , ccl3 , gene , receptor , biology , cxc chemokine receptors , chemotaxis , mast cell , immunology , genetics , ccl2
The classical pathway for mast cell degranulation is mediated through the high affinity IgE receptor FcεRI but the optimal mast cell degranulation in the conjunctiva also requires CC chemokine ligand 3 (CCL3). To examine the transcriptional changes underlying the rat basophilic leukemia mast cell line's response to costimulation of FceRI and CCL3 receptor CC chemokine receptor 1 (CCR1), we screened more than 31,000 probe sets using Affymetrix Rat Expression Array 230 2.0. Genes whose expression changed only with activation of both receptors were designated as synergy‐related genes. Among the 212 synergy‐related genes, 93 genes are functionally annotated, fourteen genes are involved in signaling pathways, 112 genes are up‐regulated while 100 genes are down‐regulated. The expression changes of the most up‐regulated synergy‐related genes including Epithelial Membrane Protein 1 (Emp1), Regulator of G protein Signaling 1 (Rgs1), and RT1 class Ib, locus S3 (RT1‐S3) were validated at both mRNA and protein levels. Their function was also analyzed by siRNA inhibition followed by degranulation and chemotaxis assays. This project is funded by MCCID EU 504926 EST program, European Commission, Brussels, Belgium.