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Interleukin‐1 facilitates recruitment of leukocytes into the skin through a complex assortment of chemokines
Author(s) -
Jensen Liselotte E
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1070.19
Subject(s) - chemokine , cxcl2 , ccl20 , cytokine , immunology , psoriasis , biology , tumor necrosis factor alpha , ccl5 , microbiology and biotechnology , medicine , inflammation , chemokine receptor , t cell , immune system , il 2 receptor
The skin constitutes an important barrier against the environment. Keratinocytes in the skin are often the first cells to encounter an intruding potential pathogen. Keratinocytes express the cytokine interleukin‐1 (IL‐1) which is a potent pro‐inflammatory cytokine. However, the specific function of IL‐1 in the skin and skin pathologies such as eczema and psoriasis remains unclear. Using pathway‐focused microarrays interrogating expression of 113 mRNAs encoding cytokines and receptors, the mRNAs for CCL5, CCL20, CXCL1, CXCL2, CXCL5, CXCL11 and IL‐8 were found to be regulated by IL‐1 in primary keratinocytes. IL‐1 dependent differential expression was confirmed using real‐time PCR and ELISA in primary and HaCaT keratinocytes. Synergy between IL‐1 and tumor necrosis factor‐alpha and interferon‐gamma was observed. These results imply that IL‐1 plays an important role in recruiting leukocytes into the skin. Further studies established that IL‐1 facilitates chemoattraction of T cells and neutrophils in the transwell system. In conclusion, IL‐1 plays a fundamental role in the dynamic interplay between keratinocytes and leukocytes aimed at establishing an effective barrier against microorganisms. This work was supported in part by the National Institutes of Health Grant AR053672, the Transdisciplinary Program in Translational Medicine and Therapeutics, and a grant from the American Heart Association.