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Determination of the functional biology of IL‐25
Author(s) -
Saenz Steven A.,
Zaph Colby,
Cua Daniel J.,
Kastelein Robert A.,
Artis David
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1069.8
Subject(s) - cytokine , biology , microbiology and biotechnology , interleukin , in vivo , cd8 , immunology , inflammation , in vitro , immune system , genetics
Interleukin (IL)‐25 (IL‐17E) is a recently described member of the IL‐17 cytokine family. Recent studies have demonstrated key functions of IL‐25 in T H 2 cytokine‐mediated host protective immunity and in the inhibition of IL‐17‐mediated inflammation. Despite a better understanding of its biological relevance, little is known about the cellular sources and targets of IL‐25 or the factors that regulate its expression. Using mice that express β‐galactosidase from the IL‐25 locus (IL25 +/lacZ mice), we identified that gut‐resident CD4 + and CD8 + T cells are the major producers of IL‐25 in vivo . To address what cytokines might drive the differentiation of IL‐25‐producing T cells, in vitro cultures of cells from IL25 +/lacZ mice were polyclonally stimulated in the presence of a variety of cytokines. Increased IL‐25 expression was observed in the presence of IL‐4, IL‐6, and TNFα. A better understanding of the regulatory pathways controlling the expression of IL‐25 may provide the rationale for targeted therapeutics for multiple inflammatory diseases.