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Functional characterization of chicken proinflammatory cytokine IL‐17D
Author(s) -
Lillehoj Hyun Soon,
Hong Yeong Ho,
Han Jae Young,
Kim Jin Kyu
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1069.11
Subject(s) - complementary dna , biology , proinflammatory cytokine , open reading frame , microbiology and biotechnology , peptide sequence , messenger rna , immunology , biochemistry , gene , inflammation
In order to characterize the proinflammatory cytokine IL‐17D, full‐length cDNA was cloned from a testis cDNA library and its biological function investigated. The full‐length chicken IL‐17D (chIL‐17D) cDNA consisted of a 348 nucleotide sequence encoding an open reading frame of 116 amino acids with a predicted molecular mass of 17.0 kDa. Comparison of the deduced amino acid sequence of chIL‐17D with homologous proteins from human, mouse and opossum revealed 64%, 53% and 76% identity respectively, including six conserved cysteine residues present in the mammalian polypeptides. The chIL‐17D gene transcript was expressed in a wide range of tissues, and highest levels were in pancreas, thymus and lung. Following Eimeria maxima infection, levels of the chIL‐17D mRNA were up‐regulated in the intestinal jejunum, bursa, lung, and spleen but decreased in the thymus. Infected chickens also expressed greater levels of chIL‐17D mRNA in CD4 + , CD8 + and TCR1 + intestinal intraepithelial lymphocytes while decreased expression was seen in TCR2 + cells. Treatment of CHCC‐OU2 fibroblasts with chIL‐17D recombinant protein induced the expression of IL‐6 and IL‐8. Collectively, these results suggest that chL‐17D has structural and functional similarities to mammalian IL‐17Ds and that it plays an important role in local gut innate immune responses during experimental coccidiosis.