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Two different human Th17 cells defined by chemokine receptor expression
Author(s) -
Sato Wakiro,
Aranami Toshimasa,
Yamamura Takashi
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1069.10
Subject(s) - c c chemokine receptor type 6 , ccr4 , cc chemokine receptors , chemokine receptor , ccr2 , chemokine receptor ccr5 , chemokine , ccl17 , immunology , microbiology and biotechnology , biology , chemistry , inflammation
Objective: IL‐17‐producing CD4+ T cells (Th17 cells) have emerged as a T cell subset that may be pivotal in the pathogenesis of autoimmune diseases. We previously reported that human Th17 cells are distinct from Th1 cells in their chemokine receptor expression; Th17 cells as CCR2+CCR5−, whereas Th1 cells as CCR5+ (J. Immunol. 178:7525–7529, 2007). But other reports demonstrated that human Th17 cells are CCR4+CCR6+. We analyzed the relationship between the two populations. Method: Healthy human PBMCs (n = 6) were stained with CCR2, CCR4, CCR5 and CCR6 as well as CD45RA and CD4 and analyzed by FACS. CCR2+CCR5− and CCR4+CCR6+ memory CD4+ T cell subpopulations were sorted by FACS. Their IL‐17‐producing capacity was measured by ELISA. Result: The frequency of CCR2+CCR5− subpopulations within memory CD4+ T cells was 2.2+/−2.0%, whereas that of CCR4+CCT6+ was 16.8+/−6.9%. 24.7+/−8.8% of CCR2+CCR5− cells were CCR4+CCR6+, whereas only 3.0+/−2.5% of CCR4+CCR6+ cells were CCR2+CCR5−. The IL‐17 production level of both subpopulations was generally similar. Conclusion: CCR2+CCR5− cells and CCR4+CCR6+ cells basically do not overlap, suggesting that each population may function at different inflammatory conditions.