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Adjuvanting of Fluzone® with JVRS‐100 in Mice, Rabbits and Non‐Human Primates Demonstrates Increased Immunogenicity and Dose‐Sparing
Author(s) -
Callejo Bernadette,
Lay Marla,
Carroll Timothy D.,
Matzinger Shan,
Fritts Linda,
Miller Christopher J.,
Chang Stella,
Warner John F.,
Fairman Jeffery
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1068.26
Subject(s) - adjuvant , immunogenicity , immune system , antigen , splenocyte , immunology , antibody , vaccination , antibody titer , hemagglutination assay , virology , titer , influenza vaccine , t cell , biology , medicine
JVRS‐100 (lipid‐DNA complexes) is a unique and promising adjuvant for vaccine applications that require high levels of antibody and T‐cell immunity. The JVRS‐100 adjuvant was mixed with a split influenza vaccine (Fluzone®‐sanofi pasteur) and administered parenterally to mice, rabbits and non‐human primates. Vaccination with JVRS‐100‐Fluzone® resulted in a significant increase in total IgG, IgG1 and IgG2a influenza antibodies. Further hemagglutination inhibiting (HAI) antibody were higher compared with Fluzone® alone. Administration of decreasing amounts of Fluzone® mixed with JVRS‐100 resulted in a ∼50‐fold dose‐sparing effect based on HAI titer. In vitro stimulation of splenocytes from JVRS‐100‐Fluzone® vaccinated mice with Fluzone® demonstrated increased antigen‐specific T cell responses (IFN‐gamma production) compared with Fluzone® alone. Splenocytes from JVRS‐100‐Fluzone® vaccinated mice responded to unmatched H1N1, or H3N2, and influenza B viruses, suggested induction of cross‐reactive T cell responses to conserved viral antigens. These results suggest the JVRS‐100 adjuvant enhanced immune responsiveness and reduced antigen doses needed for strong immune responses to a licensed flu vaccine. The JVRS‐100 adjuvant has also been shown to potentiate immune responses to multiple viral and bacterial antigens, and could be a broadly applicable adjuvant for human and veterinary vaccines.