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Modulation of Dendritic cell response by human CD8 T cells in the presence of a microbial stimulus
Author(s) -
Chong Shu Zhen
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1065.12
Subject(s) - cytotoxic t cell , priming (agriculture) , cytokine , immune system , cd8 , chemokine , microbiology and biotechnology , immunology , biology , interleukin 12 , in vitro , biochemistry , botany , germination
Dendritic cells (DCs) play a central role in the induction of immune responses by priming CD8 T cells for effector and cytotoxic functions. However, little is still known about the priming effects of CD8 T cells on DCs in the event of a microbial infection. Here, we show that human CD8 T cells are capable of modulating DC cytokine production early during the priming process. We showed that activated CD8 T cells were consistently better in activating DCs for cytokine production. Activated CD8 T cells enhanced the cytokine production of IL‐12p40, TNF‐α and GM‐CSF by DCs in the presence of a microbial stimulus. Notably, IL‐1 and IL‐12p70 required the absolute presence of both CD8 T cells and LPS. Interestingly, IL‐1 is produced by DCs with CD8 T cells but not with IFN‐g, indicating the involvement of other signals. CD8 T cells were also able to prime DCs for the production of chemokines such as RANTES and IP‐10. This study demonstrates the helper role of CD8 T cells in the enhancement of DC activity and its possible implications in immune modulation. This work is funded by the Immunology Programme (Grant no. C182‐002‐002‐001). Chong SZ is supported by the NUS Graduate School for Integrative Science and Engineering Scholarship