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Regulation of catalytic activity of S6 kinase 2 during cell cycle
Author(s) -
Joseph Alton Jude,
Boyer Derek,
Quintanilla Rene Harold,
LeeFruman Kay K
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1050.12
Subject(s) - p70 s6 kinase 1 , cell cycle , microbiology and biotechnology , pi3k/akt/mtor pathway , biology , cell , signal transduction , biochemistry
Ribosomal S6 kinase 2 (S6K2) is a homolog of S6 kinase 1 (S6K1), and both S6K1 and S6K2 are regulated by mammalian target of rapamycin (mTOR). Even though there are many similarities between the two kinases, evidence suggests that S6K1 and S6K2 may have non‐overlapping functions, but the biological function of S6K2 besides phosphorylation of S6 is yet to be determined. In order to shed light on potential cellular function of S6K2, we set out to determine cell cycle phase(s) during which S6K2 is most active. To date most research on mTOR and S6K2 had focused on G1 phase of the cell cycle since the mTOR inhibitor rapamycin blocks cell cycle progression at G1 phase. Our data shows that S6K2 is active throughout the cell cycle with higher activity in G2 and M phases, which suggests that S6K2 may have yet‐unidentified role(s) in these cell cycle phases. We are currently analyzing the signaling mechanisms that control S6K2 activity throughout the cell cycle, and we are also assessing potential regulation of S6K2 activity via sub‐cellular localization throughout the cell cycle. This research was supported by NIH SCORE Grant 2 S06 GM063119.