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Adiponectin membrane receptor 1 and 2
Author(s) -
Faile Katie,
James Alena,
Parker Chasta
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1046.2
Subject(s) - adiponectin , adiponectin receptor 1 , adipose tissue , receptor , type 2 diabetes , metabolic syndrome , endocrinology , medicine , biology , diabetes mellitus , insulin resistance
Metabolic syndrome is used to describe various health risk factors that can lead to obesity, Diabetes type 2, and cardiovascular disease. Adipose tissue plays a key role in metabolic syndrome in that it secretes many hormones and other metabolically functional molecules. One such hormone is Adiponectin (Acrp30), a 30 kDa protein hormone essential to the mediation of lipid and glucose metabolism. It has been shown that decreased levels of Acrp30 induce a higher risk for individuals to develop obesity, diabetes, and cardiovascular disease. Research on the structure and function of Acrp30 serves as an initial step in the development of novel treatment methods for obesity‐associated and vascular disorders. To complete these studies, a more in‐depth analysis of Acrp30's membrane receptors, AdipoR1 and AdipoR2, is needed. The purpose of this study is to produce high yields of recombinant Human AdipoR1 and AdipoR2 using Spodoptera frugiperda (Sf9) insect cells. Using Novagen's Insect Direct® system, AdipoR1 or AdipoR2 was transiently transfected into the Sf9 cells. The cells were then harvested and the receptors extracted using Pierce's Mem‐Per Extraction Kit. Both receptors have been successfully expressed and functionally purified. The project was supported by NIH Grant Number P20 RR‐16461 from the NCRR for support of the program entitled South Carolina IDeA Networks of Biomedical Research Excellence (SC‐INBRE) .