Premium
The Subcellular Localization of VACM‐1/CUL 5 and NEDD8 proteins in Endothelial Cells in vitro
Author(s) -
Dabney Lida R,
BurnatowskaHledin Maria A
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1043.4
Subject(s) - chemistry , microbiology and biotechnology , nedd8 , cytosol , cell cycle , kinase , subcellular localization , cytoplasm , cell , biochemistry , biology , ubiquitin , ubiquitin ligase , gene , enzyme
VACM‐1, also known as cul‐5, attenuates cellular growth by down‐regulating the production of cAMP and decreasing phosphorylation of mitogen‐activated protein kinase. Cellular localization of VACM‐1 is cell cycle‐dependent; during G 1/0 phase it localizes to the membranes, it disappears during S phase, and during M phase it localizes to the nuclear region and to the cytosol. The subcellular localization and biological activity of VACM‐1 may depend on its modification by a ubiquitin‐like protein, NEDD8. Whether NEDD8 expression is cell cycle dependent or if it co‐localizes with VACM‐1 has not been studied. This study aimed to determine whether NEDD8 expression is cell cycle dependent and if in cells transfected with VACM‐1 cDNA its expression co‐localizes with that of the VACM‐1 protein. Double‐immunofluorescent staining experiments were performed using antibodies directed against the VACM‐1 and NEDD8 proteins. Our results indicate that unlike VACM‐1 protein, NEDD8 is present through the cell cycle and co‐localizes with VACM‐1 during the M phase. These results also suggest that NEDD8 may also modify other proteins. Supported by NCI (R15CA104014), Dept of Chemistry and NSF‐URC grant No. 0629174 .