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Polyunsaturated Fatty Acids and Oxidative Stress in a Model of Hepatic Steatosis, the (fa/fa) Zucker Rat, In Vivo Effect of Chlorogenic Acid
Author(s) -
Sotillo Delcy Rodriguez,
Hadley Mary
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1034.5
Subject(s) - chemistry , medicine , steatosis , endocrinology , lipid peroxidation , polyunsaturated fatty acid , malondialdehyde , oxidative stress , chlorogenic acid , fatty liver , fatty acid , biochemistry , biology , food science , disease
Altererations in lipid metabolism gives rise to hepatic steatosis, due to increased lipogenesis affecting polyunsaturated fatty acids (PUFA). Excessive formation of reactive oxygen species is associated with the onset and progression of steatosis. Previously, we reported that chlorogenic acid (CGA), a phenolic antioxidant, decreased plasma and liver triacylglycerols in Zucker ( fa/fa ) rats, a model of insulin resistance and steatosis. This study aimed to investigate the in vivo effect of CGA on lipid peroxidation in Zucker ( fa/fa ) rats. Rats were implanted with jugular vein catheters and CGA was infused (5 mg/Kg body weight/day) for 3 weeks. Analysis were carried out when rats reached 22 weeks old of age. Malondialdehyde (MDA), a biomarker of oxidative stress, was determined in plasma, urine, liver, and kidney. Fatty acid methyl esters were analyzed in liver and adipose tissues. Chlorogenic acid treatment decreased MDA in plasma by 8%; in urinary by 35%, and in liver by 18.4 % compared to control rats. No differences in MDA concentration due to CGA treatment were found in the kidney (p>0.05). Chlorogenic acid decreased the concentration of plasma non‐esterified fatty acids and in the liver increased cocentrations of 18:2 (n‐6), 18:3 (n‐3), and 20:4 (n‐6) were found when compared to control (p<0.05). Chlorogenic acid decreased lipid peroxidation and the ratio of n‐6:n‐3 fatty acids in an animal model of hepatic steatosis.