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Ski is required for normal development of embryonic progenitors of hypaxial muscle
Author(s) -
Zhang Hong,
Chen Yishi,
Colmenares Clemencia,
Stavnezer Edward
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1029.8
Subject(s) - progenitor cell , pax3 , microbiology and biotechnology , embryonic stem cell , progenitor , biology , regulator , anatomy , myocyte , stem cell , gene , genetics , transcription factor
The Ski encodes a transcriptional co‐regulator that stimulates muscle differentiation. We have found that Ski−/− embryos exhibit selective defect in hypaxial muscles. Hypaxial muscles are derived from progenitor cells that originate in the dermomyotome, migrate to distal sites and commit to a myogenic fate. However, the Ski−/− embryos showed that the dermomyotome developed normally. We found that myogenic determination and terminal differentiation were initiated appropriately in the absence of Ski . However, the number of cells expressing myogenic regulatory factors (MRF) genes was reduced and they were restricted to proximal limbs. Our study shows that this deficiency in number and distribution of MRF + cells is not caused by abnormal proliferation, apoptosis or differentiation. Further examination of Pax3 and Met , which are important for progenitor cell migration, indicated that this defect resulted from impaired migration of myogenic progenitors. This work was supported by NIH grants (DE15198 to C.C and E.S, CA43600 to E.S, and HD30728 to C.C).