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Cloning and expression of a putative metacaspase gene from Schizophyllum commune
Author(s) -
Carrese Alexander James,
Fox Melissa M.,
Fox Kristin M.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1003.12
Subject(s) - biology , complementary dna , schizophyllum commune , gene , genetics , cdna library , genomic dna , open reading frame , genomic library , cloning (programming) , molecular cloning , microbiology and biotechnology , peptide sequence , biochemistry , computer science , programming language
Caspases are essential players in mammalian apoptosis, but their role in the fungal life cycle is not well understood. If the role of apoptosis can be examined in simpler organisms, hopefully it will aid in elucidation of the role of the details of this process in overall development. Using degenerate PCR based on the sequence of the yeast metacaspase, YCA1, a putative metacaspase has been identified in S. commune , and its genomic sequence has been determined. Because the entire open reading frame is not present in the cDNA library, it is being constructed through splicing by overlap extension PCR with the genomic DNA and the partial cDNA clone as templates. At this point, the cDNA has been successfully amplified. Further work will involve amplifying three missing exons and constructing the full gene.