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Identification of novel regulators of growth and ARF tumor suppressor signaling
Author(s) -
Smith Tarik Jamar,
Tompkins Van,
Hagen Jussara,
Fitzgerald Matthew,
Domann Frederick,
Eischen Christine,
Lushnikova Tamara,
Quelle Dawn
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb84-c
Subject(s) - suppressor , mdm2 , biology , microbiology and biotechnology , rna interference , signal transduction , cell growth , function (biology) , protein–protein interaction , nuclear protein , interactor , cancer , cancer research , computational biology , rna , genetics , gene , transcription factor
The ARF Tumor Suppressor is a nucleolar protein that protects us against cancer through protein‐protein interactions. ARF inhibits growth by activating partially defined p53‐dependent and p53‐independent signaling pathways. To further define those pathways we preformed a yeast two hybrid interactive screen using ARF as bait and identified several new ARF partners. Notably, two of the ARF binding proteins we discovered are novel proteins with no known function. We found that both proteins are growth inhibitors that assist ARF in suppressing cell proliferation, and we named the proteins NIAM (Nuclear Interactor of ARF and Mdm2) and Parf (Partner of ARF). Our finding suggest NIAM and Parf are novel tumor suppressors, and as such they represent potential targets of new anticancer therapies. Current and future studies are aimed at testing the role of NIAM and Parf in tumor suppression using a variety of complementary approaches, including RNA interference and mouse models of cancer.