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Vitamin C regulates differential gene expression in lymphocytes and monocytes isolated from healthy women
Author(s) -
Wang Jin,
Chen Weiping,
Poy George,
Wang Yaohui,
Cam Margaret C,
Levine Mark
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb77-c
Subject(s) - gene expression , vitamin , signal transduction , gene , cd28 , vitamin d and neurology , vitamin c , calcitriol receptor , biology , messenger rna , immunology , monocyte , endocrinology , medicine , microbiology and biotechnology , t cell , immune system , biochemistry
Vitamin C is accumulated in human lymphocytes and monocytes, but its function is unknown. We hypothesized that vitamin C might regulate gene transcription in these cells. To investigate, we studied lymphocytes and monocytes isolated by apheresis from 11 healthy women 19–27 years old. Cells were isolated from the same women at steady state at each of the following daily vitamin C doses: 30mg; 60 mg; 100 mg; and 1000 or 2500 mg. Comparisons to determine significant changes in gene expression were performed using mRNA from the same subjects at different doses, with RNA obtained at 30 mg as baseline. Gene expression was analyzed using Affymatrix arrays, statistical and pathway software (GeneSpring © , Partek © , GeneGo © ), and confirmed by quantitative real time PCR. The data showed that in monocytes higher Vitamin C doses significantly increased expression of multiple components of three similar pathways: CD28 signaling; ICOS‐ICOSL signaling; and T cell receptor signaling. Also, in monocytes the EGF signaling pathway was significantly increased with increasing vitamin C doses. In lymphocytes, fewer pathways were affected compared to monocytes. These analyses reveal novel functions of vitamin C on gene transcription in circulating cells in human plasma. These are the first data describing changes in gene expression as a function of varying nutrient concentrations in the same human subjects, for any nutrient.