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In vivo cytokine profiling in peripheral arterial disease (PAD)
Author(s) -
Girn H. R . S .,
Orsi N.M.,
HomerVanniasinkam S.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb71-b
Subject(s) - cytokine , medicine , in vivo , ex vivo , multiplex , disease , immunology , bioinformatics , biology , microbiology and biotechnology
Objective: This study determined Th1:Th2‐type cytokine balance in relation to PAD severity. Methods: Following ethics approval, plasma was isolated from 10 controls, 14 stable claudicants (SC), 14 critical ischaemics (CLI). PAD diagnosis included Fontaine classification, ABPI and radiology. Th1 (interleukin (IL)‐6, IL‐12(p70), interferon (IFN)‐γ) and Th2 (IL‐10) cytokine profiles were measured by multiplex immunoassay. Results: IL‐6 levels tended to increase in PAD, primarily with CLI, in contrast to IL‐12(p70). IL‐6:IL‐10 ratio was higher in PAD, particularly with CLI, as opposed to IL‐12(p70):IL‐10 ratio (P<0.05). PAD had no effect on IFN‐γ:IL‐10 ratio. Conclusion: Estimation of Th1:Th2 cytokine ratios probably provide a more sensitive measure of disease progression and are more representative of in vivo cytokine synergism. Project supported by LGI vascular surgical research fund. 1 Cytokines and ratios in PAD (different superscripts = significant differences between groups/∗significantly different to controls; P<0.05)