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Hydroxynonenal in Obstructed Urinary Bladder Disease
Author(s) -
Hass Martha A.,
Levin Robert M.,
SiflingerBirnboim Alma
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb7-b
Subject(s) - lipid peroxidation , 4 hydroxynonenal , urinary system , chemistry , urinary bladder , blot , lipid oxidation , immunoassay , medicine , endocrinology , urology , biochemistry , enzyme , antibody , immunology , antioxidant , gene
Elevated levels of oxidized proteins have been observed in urinary bladder tissues subjected to partial outlet obstruction. 4‐Hydroxynonenal (4‐HNE), a toxic end‐product of lipid peroxidation, has been implicated in oxidative modification of proteins and enzymes. The objective of this study was to measure the levels of 4‐HNE in obstructed urinary bladder tissue and to determine if 4‐HNE oxidizes bladder proteins. Partial bladder outlet obstructions (POO) were performed on male New Zealand White (NZW) rabbits and bladders were excised, weighed and evaluated at 1, 3, 7, and 14 days. 4‐HNE was extracted from bladder tissues as its oxime or methoxime and analyzed by thin layer chromatography (TLC) and gas chromatography. Slot blot immunoassay using anti‐DNPH antibodies were used to measure protein oxidation. Elevated levels of protein oxidation and 4‐HNE were observed in obstructed bladder tissues relative to controls. Incubation of 4‐HNE with bladder proteins resulted in elevated levels of protein oxidation consistent with those observed during POO. 4‐HNE, a product of lipid peroxidation is generated during POO and contributes to protein oxidation that occurs during POO.