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Glioblastoma stem cells produce angiogenic factors through activation of chemokine receptor CXCR4
Author(s) -
Bian Xiuwu,
Yao Xiaohong,
Ping Yifang,
Chen Jianhong,
Wang Ji Ming
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb67-a
Subject(s) - angiogenesis , cxcr4 , cancer research , stem cell , u87 , neovascularization , glioma , cancer stem cell , chemokine receptor , endothelial stem cell , biology , chemokine , immunology , microbiology and biotechnology , in vitro , inflammation , biochemistry
Tumor stem cells have been shown to be critical for tumor formation, angiogenesis and progression. In order for a better understanding of the role of tumor stem cells in angiogenesis, we isolated CD133+ glioma stem cells from a human glioblastoma cell line U87 and investigated their capacity to produce angiogenic factors. We found that CD133+ stem cells from U87 cell line produced increased levels of vascular endothelial cell growth factor (VEGF) and interleuikin 8 (IL‐8/CXCL8), two important angiogenic factors implicated in human malignant tumors. CD133+ glioblastoma stem cells also expressed chemokine receptor CXCR4, and upon stimulation with the ligand SDF‐1 (CXCL12), markedly enhanced the production of both VEGF and CXCL8. In nude mice, CD133+ glioblastoma stem cells formed larger xenograft tumors with more vigorous neovascularization, in addition to exhibiting the capacity of self‐renewal. Our results suggest that CD133+ human glioblastoma stem cells may play an active role in tumor angiogenesis through the CXCR4/CXCL12 interaction. (This project was supported by grants from National Natural Science Foundation of China, No. 30670804, and the National 973 Program, No.2006CB708503)