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Evaluation of a novel tissue transport media for muscle biopsy specimens
Author(s) -
Sandberg Glenn D.,
HorkayneSzakaly Iren,
Rushing Elisabeth J.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb66-c
Subject(s) - biopsy , muscle biopsy , stain , medicine , pathology , muscle tissue , needle biopsy , staining , anatomy
Study objective: Muscle biopsy plays an integral role in the diagnosis and management of neuromuscular diseases. Routine histochemical stains performed on frozen muscle biopsy specimens are subject to artifacts during the procedure and/or transport that may hinder interpretation. Our goal is to evaluate whether Liforlab™, a high oxygen capacity transport solution preserves enzymatic activity in fresh muscle biopsies. Materials and methods: Using pig muscle, we processed 9 fresh and a single formalin fixed biopsy specimen. A panel of 8 enzyme histochemical stains and one MHC1 immunohistochemical stain were performed on all fresh biopsy specimens; an additional dystrophy panel was performed on 2 cases. Results: Liforlab™ preserved enzymatic activity and immunoreactivity in fresh muscle biopsy tissues up to 144 hours (5 days). Compared to the standard frozen biopsy specimens, biopsies processed with this agent contained fewer artifacts. Conclusion: Our results are promising and warrant further evaluation of this media on human muscle biopsy specimens.