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Isotopic profile of intramyocellular fatty acid pools in human skeletal muscle
Author(s) -
Guo ZengKui,
Kanaley Jill,
Sheehan Michael,
Jensen Michael
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb43-c
Subject(s) - nefa , skeletal muscle , triglyceride , medicine , fatty acid , chemistry , endocrinology , biochemistry , biology , cholesterol
Fatty acid kinetics in skeletal muscle is not well understood. [U‐ 13 C]palmitate (0–6 h) and [U‐ 13 C]oleate (5–11 h) were infused at 3 nmol·kg −1 ·min −1 in resting healthy lean and obese men and women (n=4) and blood and muscle samples collected after each tracer infusion (t 0 , t respectively). Palmitate and oleate 13 C enrichments in plasma and muscle non‐esterified fatty acid (NEFA), triglyceride (imcTG) and acylcarnitine (AC) pools were determined. The two tracers showed similar patterns across these pools but intra‐ vs. extracellular ratio is higher for oleate than palmitate confirming greater oleate uptake. Both tracers had similar enrichments in NEFA and AC but higher than in imcTG indicating kinetic distinction among these pools. Support: NIH DK 40484.