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Inhibitory effects of roasted licorice on high glucose‐induced cytotoxicity in human renal mesangial cells
Author(s) -
Park EunHee,
Choi YeanJung,
Choi JungSuk,
Kang SangWook,
Bae JiYoung,
Kang YoungHee
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb42-b
Subject(s) - mesangial cell , cytotoxicity , chemistry , diabetic nephropathy , nephropathy , mannitol , nadph oxidase , endocrinology , medicine , pharmacology , diabetes mellitus , biochemistry , reactive oxygen species , in vitro
Hyperglycemia appears to be an important causative factor in the development of vascular complications in patients with diabetes. It has been shown that NADPH oxidases play a potential role in the pathogenesis of diabetic nephropathy. This study examined the inhibitory actions of dry‐roasted licorice ethanol extracts (rLE) in diabetic nephropathy. Human renal mesangial cells (HRMCs) were treated with 1–20 μg/ml rLE and exposed to normal (5.5 mM) and high (33 mM) glucose media for 5 days. Osmotic control cells were incubated with 27.5 mM mannitol in presence of 5.5 mM glucose. It was shown that a 5‐day incubation of high glucose induced mesangial cytotoxicity and rLE inhibited this cytotoxicity in a dose‐dependent manner at ≥1 μg/mL. Chronic high glucose‐reduced cell viability was improved by non‐polar fractions of rLE at ≥1 μg/mL. Western blot analysis revealed that chronic high glucose induced activation of NADPH subunits of p22phox and p47phox. rLE and its non‐polar fractions substantially attenuated the activation of these mesangial NADPH oxidases. These results suggest that the blockade of renal mesangial cytotoxicity by non‐polar fractions of rLE was most likely mediated by a reduction of oxidant production through their interference with high glucose‐induced activation of NADPH oxidases. The detailed mechanisms involved in inhibition of diabetic nephropathy by roasted licorice should be further elucidated. Supported by the Korea Science and Engineering Foundation (R01‐2006‐000‐10896‐0).