Radical Scavenging Effect and Anti‐inflammatory Activity of Aureobasidum sp. β‐glucan in LPS‐treated RAW 264.7 macrophage

Aureobasidum sp. β‐glucan is glucose homopolymer with β‐1,3 glycosidic bond and dietary fiber with high viscosity and solubility. This is prescribed for anti‐cancer and anti‐inflammatory effect. It has been known to act as an antioxidant which is relates to diabetes. The purpose of this study was to investigate the effects of the extracts of Aureobasidum sp. β‐glucan on the anti‐oxidant and anti‐inflammatory activity in lipopolysaccharide‐stimulated RAW 264.7 macrophage. Inhibition of NO production was observed when cells were cotreated with fractions of Aureobasidum sp. β‐glucan and lipopolysaccharide. High amount of nitric oxide (NO) from iNOS and high amount of PGE2 derived from COX‐2 induced by many pro‐inflammatory mediators including TNF‐α, IL‐1β, and LPS has been implicated in the pathogenesis of sepsis and inflammation. The anti‐iflammatory effect of Aureobasidum sp. β‐glucan is associated with the induction of NO, PGE2, TNF‐α, and IL‐1β in lipopolysaccharide‐stimulated RAW 264.7 macrophage. Therefore, the results obtained from this study provide an alternative protective mechanism of Aureobasidum sp. β‐glucan and provide information on the potential use of Aureobasidum sp. β‐glucan chemoprevention or pathogenic conditions related to overproduction of NO and PGE2. However, the mechanism of the inflammatory effect must be evaluated through various parameters for induction of NO production. These results indicate that Aureobasidum sp. β‐glucan has radical scavenging effect and anti‐inflammatory effect.