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Isolation of Acetylcholinesterase Inhibitors from the Flowers of Chrysanthemum indicum Linne
Author(s) -
Jeung SeungKyoung,
Yim Hyun Jae,
Kim Hye Mi,
Shin Se Gye,
Han Seoung Jun,
Park Jung HY,
Kang IlJun
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb39-d
Subject(s) - acacetin , acetylcholinesterase , aché , apigenin , chemistry , luteolin , tacrine , carthamus , traditional medicine , biochemistry , flavonoid , enzyme , antioxidant , medicine
There have been interests in finding new sources of acetylcholinesterase (AChE) inhibitors to treat Alzheimer’s disease, because only few AChE inhibitors such as galanthamine, physostigmine, and tacrine are currently available in clinical use. In the present study we observed that the ethanol extract of the flowers of Chrysanthemum indicum Linne markedly decreased AChE activity. Using a bio‐guided fractionation strategy we identified acaciin and acacetin‐7‐O‐β‐D‐galactopyranoside as active compounds responsible for the AChE inhibition. In addition, we investigated the relationship between structure and activity with five flavonoids (acaciin, acacetin‐7‐O‐β‐D‐galactopyranoside, luteolin, and two other commercially available flavonoids, i. e . apigenin and acacetin) and observed that the presence of methoxy groups at C‐4′ and a sugar at O‐7 in ring A was essential for the inhibition of AChE. The present results suggest that acaciin and acacetin‐7‐O‐β‐D‐galactopyranoside from the flowers of Chrysanthemum indicum Linne have potential as therapeutic agents for Alzheimer’s disease.