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Bone mineral density of engineered bone constructs before and after implantation
Author(s) -
Smietana Michael J,
Wellington Michael,
Arruda Ellen M,
Larkin Lisa M
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb3-c
Subject(s) - bone mineral , medicine , femur , fetus , stromal cell , tissue engineering , bone density , bone marrow , bone tissue , andrology , anatomy , biomedical engineering , surgery , osteoporosis , pregnancy , biology , genetics
Engineered bone tissues are viable options for repair of tissue damaged by disease or extensive trauma. Our laboratory has developed an in vitro engineered bone construct (EBC) using bone marrow stromal cells (BMSC). The purpose of this project was to analyze the bone mineral density (BMD), using DEXA, of EBCs before and after 4 wks of implantation and compare the explanted constructs to normal rat bone at different developmental stages. BMSC from rat femur were cultured in medium that induced osteogenic differentiation. After reaching confluence, the tissue monolayer of the cells contracted around two constraint points forming scaffoldless cylindrical EBCs. The EBCs were placed in silicone tubing 10 mm x 1.6 mm and implanted into the hindlimb of a rat. Following 4 wks of implantation, the EBCs were removed from the animal and tested for BMD. The femur was extracted from Fisher 344 rats and BMD determined in: E‐20 fetal pups; day 4, 7, 14 and 21 day neonates. Negligible BMD was observed in the constructs prior to implantation and in E‐20 fetal pups. After 4 wks, an increase in BMD was seen in the engineered constructs (0.086 g/mm 3 ). BMD of our EBCs, while greater than that observed in fetal and day 7 neonatal, is 16% of that found in adult bone (0.507 g/ mm 3 ).Therefore, our EBCs may be viable options for use in bone replacement therapy. Supported by DARPA AFOSR FA9550‐05‐1‐0015.